What is M9 medium?

M9 minimal medium (M9) is commonly used for the cultivation of bacteria (Miller, et al., 1972). It is comprised of a M9 minimal salts base formulation that can be supplemented with various amino acids and carbon sources.

How do you make a M9 medium?

For 50 ml stock solution dissolve 50 mg thiamin-HCl in 45 ml water. Add water to a final volume of 50 ml. Sterilize the solution over a 0.22-µm filter. Prepare 1 ml aliquots and store at -20°C.

Can E coli grow on M9?

E. coli cultures were first grown aerobically at 37 °C in M9 medium supplemented with 0.2% glycerol.

How do you make a M9 agar plate?

Agar. Add 15 gm Bacto-agar to 500 mls Milli-Q water in 2 liter erlenmeyer flask, add a medium size stir bar, and cover with Al-foil. Autoclave M9 Salts and Agar solutions using the 20-minute liquid cycle and after autoclaving, place in a water bath set at 55 °C.

Can you autoclave M9?

Filter-sterilize and store at 4°C. Autoclave and store at room temperature.

Can you autoclave M9 media?

What is minimal salt medium?

General description. M9 Minimal Salts is a highly-referenced microbial growth medium used for the cultivation of E. coli. This buffered minimal microbial medium contains only salts and nitrogen, so it is traditionally supplemented with glucose, amino acids and vitamins as needed.

What are M9 salts?

M9 Minimal Salts is a highly-referenced microbial growth medium used for the cultivation of E. coli. This buffered minimal microbial medium contains only salts and nitrogen, so it is traditionally supplemented with glucose, amino acids and vitamins as needed.

What is minimal media agar?

M9 minimal medium (M9) is commonly used for the cultivation of bacteria. It is comprised of a M9 minimal salts base formulation that can be supplemented with various amino acids and carbon sources. Teknova offers various formulations of the M9 medium with different additives.

What is minimal medium in microbiology?

A culture medium for microorganisms that contains the minimal necessities for growth of the wild-type. A medium containing only inorganic salts, a carbon source, and water.

How do you make minimal salt medium?

To prepare minimal medium, add 200 ml sterile 5X Minimal Salts to 750 ml sterile distilled water. Aseptically add 20 ml filter sterilized 20% glucose solution and 2 ml sterile 0.1 M Magnesium sulphate (MgSO4) solution. If desired, add sterile 0.1 ml of 1.0 M Calcium chloride solution or amino acids as required.

What is the use of M9 minimal medium?

M9 minimal medium. This medium is used to grow B. subtilis in the presence of glucose or other variable carbon sources. The medium does contain ammonium and is therefore not suited to change the nitrogen source.

What is the minimum amount of M9 to add to the reagent?

M9 minimal medium (standard) Reagent Amount to add (for 100 mL) M9 salts (5X) 20 mL Glucose (20%; Sigma-Aldrich) a 2 mL MgSO 4 (1 M; Fisher Scientific) b 200 μL CaCl 2 (1 M; Fisher Scientific) b 10 μL

How do you prepare M9 media for chromatography?

Preparation of the medium. For 1 liter of M9 medium add in the following order: 200 mL M9 5x stock (sol. C) 1 mL 100 mM CaCl2 (sol. E) 10 mL trace salts 100x stock (sol.D) 1 mL 1 M MgSO4 (sol.

How to find the reference angle for degrees?

How to find the reference angle for degrees. All you have to do is follow these steps: Choose your initial angle – for example, 610°. If your angle is larger than 360° (a full angle), subtract 360°.