What is the principle of India ink preparation?

Principle of Negative Staining India Ink or Nigrosin is an acidic stain. This means that the stain readily gives up a hydrogen ion (proton) and the chromophore of the dye becomes negatively charged. Since the surface of most bacterial cells is negatively charged, the cell surface repels the stain.

What technique is used in the identification of Cryptococcus neoformans in CSF?

The PCR was easy to perform and needed only 4 h for all processes from receiving the CSF to detection of a specific DNA band after agarose gel electrophoresis. This would provide another rapid laboratory method for the diagnosis of cryptococcal meningitis.

What is the importance of India ink staining in diagnosis of infection?

Comparism of fungal culture by Indian ink staining. Whereas Indian ink stain is useful as a screening test, it has a low confirmatory test value for CM in patients with HIV and may lead to over-diagnosis of CM. Positive Indian ink test should be confirmed by culture.

Why is India ink used for Cryptococcus?

A novel modified India ink technique for the diagnosis of Cryptococcus neoformans in cerebrospinal fluid specimens is described. It employs 2% chromium mercury and India ink. This technique allows a clear identification of some external and internal structures of the organism.

What is India ink stain used for?

Microbiologists use India ink to stain a slide containing micro-organisms. The background is stained while the organisms remain clear. This is called a negative stain. India ink, along with other stains, can be used to determine if a cell has a gelatinous capsule.

What is the purpose of India ink used for in microbiology?

India ink capsule stain is used to demonstrate cell capsules through microscopic examination. This procedure is used to detect presence of encapsualted species, e.g. Cryptococcus neoformans, which causes cryptococcosis in humans.

How is Cryptococcus neoformans identified?

Currently, identification of Cryptococcus to the genus or subgenus level from clinical specimens relies upon the microscopic examination of yeast cells in conjunction with biochemical tests, differential media, and/or DNA sequence analysis (9, 22, 23, 31, 33, 44–46).

What’s mean India ink stain preparation of CSF?

What is another name used for India ink?

India ink, also called Chinese Ink, black pigment in the form of sticks that are moistened before use in drawing and lettering, or the fluid ink consisting of this pigment finely suspended in a liquid medium, such as water, and a glutinous binder.