What color would the M smegmatis look after the acid-fast stain?
What color is Mycobacterium smegmatis in an acid fast stain? Why? It is red because it is acid fast positive. The carbolfuchsin is more soluble in the cell wall lipids therefore, retains the red color.
What is the acid-fast reaction of Mycobacterium smegmatis?
smegmatis was acid-fast positive. The acid-fast stain is classified under differential staining procedures that are used to pick out acid-fast organisms, including bacteria of the genus Mycobacterium. These organisms have a thick, waxy almost impervious cell wall made up of mycolic acid and complex lipids.
Is Mycobacterium smegmatis acid-fast positive?
The small pink bacilli above are Mycobacterium smegmatis, an acid fast bacteria because they retain the primary dye.
What is the morphology and arrangement of Mycobacterium smegmatis?
Mycobacterium smegmatis is an acid-fast bacterial species in the phylum Actinomycetota and the genus Mycobacterium. It is 3.0 to 5.0 µm long with a bacillus shape and can be stained by Ziehl–Neelsen method and the auramine-rhodamine fluorescent method.
What is the colony morphology of Mycobacterium smegmatis?
The surface morphology of this microbe will be shiny, smooth, finely wrinkled or coarsely collapsed . M. smegmatis is found to non-pathogenic and rapidly developing than other Mycobacterium species . The organism is aerobic, which donates the final electrons to oxygen during respiration.
What is the color of Mycobacterium smegmatis?
When Mycobacterium smegmatis has been growing for quite some time (generally after 48 hr growth) and is abundant, the color will turn from white to a nonpigmented creamy yellow. It will also be waxy because of the high amount of unique Gram-positive cell wall coated with mycolic acids.
Is Mycobacterium smegmatis catalase positive or negative?
Biochemical characteriza- tion of the isolate showed that it was catalase, glucose, sucrose, mannitol and nitrate positive reduction and was negative for maltose, CAMP test, oxidase and aesculin. The isolate has in vitro similar pattern to M. smegmatis as similar previously reported [5,8].
Is Mycobacterium smegmatis Gram-positive or negative?
Mycobacterium smegmatis is a Gram-positive bacteria, characterized by an inner cell membrane and a thick cell wall.
What color did the Mycobacterium stain on your slide?
Those cells which retained the primary stain (carbol fuchsin) through the acid-alcohol treatment are stained red; these are the acid-fast organisms. Mycobacterium cells characteristically appear as clusters of long, red rods.
Is Mycobacterium smegmatis shape and arrangement?
What are the characteristics of Mycobacterium smegmatis?
Mycobacterium smegmatis is a Gram-positive bacteria, characterized by an inner cell membrane and a thick cell wall. The Gram-positive bacteria is further classified as one with a high GC content and therefore a low AT content. This quality is used as a crude measure of similarity of different species of bacteria.