What does 2-aminopurine DNA do?
2-aminopurine is able to form Watson–Crick base pair with thymine, so it maintains the structure of B-form of DNA. An oligonucleotide is synthesized with a regular incorporation of multiple number of 2-aminopurine, which is then hybridized with a complementary strand to form a double-stranded substrate.
What is base analog mutagen give example?
Base analogs are mutagenic chemicals that can be substitute for purines or pyrimidines during nucleic acid biosynthesis. For example, the nucleoside analog bromodeoxyuridine (BrdU) is formed when 5-bromouracil (5-BU) is chemically linked to deoxyribose (Fig. 2.23).
What does 5-Bromouracil do to DNA?
5-Bromouracil (BrU) is a base analogue of thymine (T) which can be incorporated into DNA. It is a well-known mutagen, causing transition mutations by mispairing with guanine (G) rather than pairing with adenine (A) during replication.
What type of mutation will be induced by adenine analog 2 aminopurine?
During subsequent replication, this causes AT-to-GC transition mutations. 2-AP is an adenine analog and it usually pairs with thymine, but it can also form pairs with cytosine, causing both AT-to-GC and GC-to-AT transition mutations.
How does ethyl methanesulfonate work?
Ethyl methanesulfonate (EMS) is a mutagenic, teratogenic, and carcinogenic organic compound with formula C3H8SO3. It produces random mutations in genetic material by nucleotide substitution; particularly through G:C to A:T transitions induced by guanine alkylation. EMS typically produces only point mutations.
Are alkylating agents mutagens?
The most commonly used chemical mutagens are alkylating agents such as ethylmethane sulfonate and N-methyl-N-nitrosourea that induce point mutations in DNA.
What is Tautomer of 5 bromo uracil?
5-Bromouracil (5-BrU, 5BrUra, or br5Ura) is a brominated derivative of uracil that acts as an antimetabolite or base analog, substituting for thymine in DNA, and can induce DNA mutation in the same way as 2-aminopurine.
What is ethyl methanesulfonate mutagenesis?