What does a Myc tag do?
The Myc tag, derived from the c-Myc protein, is a popular epitope tag for detecting the expression of recombinant proteins in yeast, bacteria, insect, and mammalian cell systems. The Myc tag may be fused to either the N-terminus or C-terminus of a protein.
How many amino acids are in Myc tag?
10 amino acids
The Myc-tag contains 10 amino acids (aa) and the sequence is EQKLISEEDL.
What is anti c-myc?
Anti-c-myc is a monoclonal antibody to c-myc peptide (clone 9E10). Anti-c-myc recognizes the 9E10 epitope sequence (EQKLISEEDL), derived from the human c-myc protein. The monoclonal antibody against the c-myc epitope is well characterized and does not cross-react with other cellular proteins.
How many kDa is Myc?
The Myc tag can be used to detect recombinant proteins in bacteria, yeast, insect and mammalian cell systems. Its short peptide sequence is N-EQKLISEEDL-C and has a size of about 1.2 kDa.
What DNA structure does C-MYC bind in DNA?
The c-Myc protein is a potential activator of transcription, with the ability to bind in a heterodimer form with Max to DNA sequences containing the core hexanucleotide sequence CAC(G/A)TG.
What is C-myc epitope?
What is c-Myc epitope?
How much does Myc tag weigh?
c-Myc tag peptide is a synthetic peptide with a molecular weight of 1,203 Da. The peptide sequence is EQKLISEEDL, corresponding to the C-terminal amino acids (410-419) of human c-myc protein.
What is the difference between MYC and c-myc?
Myc and c-myc are the same thing, the normal cellular (hence the c) version of this protein, which can be oncogenic when mutated. The myc tag is a 10-residue sequence derived from c-myc, which is a much larger protein.
What is the difference between c-myc and N-Myc?
c-Myc is broadly overexpressed in both blood-borne and solid tumors. N-myc is most frequently overexpressed in solid cancers of neural origin, such as neuroblastoma and glioma. And L-myc is most often overexpressed in small cell lung carcinomas.