What is Capto Q resin?

Capto Q is designed for capture and intermediate purification of proteins from large feed volumes. It is composed of a rigid, high-flow agarose matrix modified with dextran surface extenders and a strong quaternary ammonium (Q) anion exchanger.

What is Capto MMC?

CaptoŒ MMC is a multimodal salt tolerant BioProcessŒ medium for capture and intermediate purification of proteins from large feed volumes by packed bed chromatography. Capto MMC increases productivity and reduces cost with: ‡ high dynamic binding capacity at high conductivity. ‡ high volume throughput.

What is Q Sepharose?

Q Sepharose® Fast Flow is a strong anion exchanger based on the well established Sepharose® Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

How does anion exchange chromatography work?

Anion exchange chromatography is a form of ion exchange chromatography (IEX), which is used to separate molecules based on their net surface charge. Anion exchange chromatography, more specifically, uses a positively charged ion exchange resin with an affinity for molecules having net negative surface charges.

How does Q Sepharose work?

Two strong exchangers are Q-Sepharose Fast Flow and SP-Sepharose Fast Flow. The charged group of Q-Sepharose is a quarternary amine which carries a non- titratable positive charge. This matrix can be used at alkaline pH values at which the positive charge of the DEAE group would have been titrated.

What are resins in ion exchange method?

An ion-exchange resin or ion-exchange polymer is a resin or polymer that acts as a medium for ion exchange. It is an insoluble matrix (or support structure) normally in the form of small (0.25–1.43 mm radius) microbeads, usually white or yellowish, fabricated from an organic polymer substrate.

How do you clean Q Sepharose?

you can easily clean Q sepharose resin with 4-5 culun volume of 2M NaCl then followed by 8M Urea or 7M guanidium hydrochloride and excess for distilled water and try to store your resin with at least 20% ethanol for long term storage. You can never fully clean Q sepharose.