What is indophenol blue method?
The indophenol blue (IPB) method based on Berthelot’s reaction is one of the most widely used methods for the determination of ammonium in natural waters. This study comprehensively optimized the kinetics of the IPB reaction under different reagent concentrations, temperature and salinity.
What is Indophenol method?
The indophenol method for determining ammonia in water samples is based on the formation of an indophenol blue pigment during the reaction of phenol and hypochlorite in the presence of ammonia. In alkaline medium (pH = 8–11.5) a chloramine is first produced.
How is Indophenol formed?
In this method, the ammonia generated is converted into chloramine on reaction with a sodium hypochlorite solution, which then reacts with phenol to produce the blue-colored indophenols.
How do you analyze ammonia in water?
Ammonia is analyzed by chemical titration. The method used in most test kits is called the salicylate method. Always measure pH and temperature when you measure ammonia. Without these other measurements it will be difficult to know the toxicity of the ammonia.
How do you make Indophenol solution?
Fun Fact: You can make an indophenol solution by mixing 0.5 g of indophenol powder with 1 cup of water.
Under what chemical conditions does Indophenol appear clear?
When a liquid containing Vitamin C is added to Indophenol Solution, the colour changes from Blue to Clear.
How do you make indophenol dye?
Dye solution: Dissolve 50 mg of the Sodium salt of 2, 6 dichlorophenol indophenol dye in 150 ml hot glass distilled water containing 42 mg of Sodium bicarbonate. Cool and dilute with distilled water to 200 ml. Store in refrigerator and standardize.
Under what chemical conditions does indophenol appear clear?
What is used to measure ammonia?
Ammonia nitrogen can be measured using a spectrophotometer (or colorimeter) or an ammonia ion selective electrode (ISE).
What causes Indophenol to change from blue to clear?
Vitamin C reacts with the indophenol and causes its color to change. A liquid containing a lot of vitamin C needs just one drop to cause all the indophenol to react.