What is Merrifield solid phase peptide synthesis?
Merrifield solid phase peptide synthesis has been the principle research procedure used in the study of the chemistry and biological use of deamidation of asparaginyl and glutaminyl residues in peptides and proteins during the past 40 years.
What is solid phase protein synthesis?
Solid-phase peptide synthesis (SPPS) involves the successive addition of protected amino acid derivatives to a growing peptide chain immobilized on a solid phase, including deprotection and washing steps to remove unreacted groups and also side products.
Who developed the process of solid phase synthesis for peptide chains?
R. B. Merrifield
Peptide containing up to 80 amino acid residues can be synthesized in reasonable yield. R. B. Merrifield was awarded the Nobel Prize in Chemistry in 1984 for inventing solid state peptide synthesis.
How are polypeptides synthesized?
Polypeptide synthesis is the biological production of peptides, which are organic compounds in which multiple amino acids are linked via peptide bonds. They are the building blocks of proteins, which are essential to cell function. The process involves two steps: transcription and translation.
What is solid phase peptide synthesis used for?
The concept of solid-phase peptide synthesis (SPPS) is to retain chemistry that has been proven in solution but to add a covalent attachment step that links the nascent peptide chain to an insoluble polymeric support (resin). Subsequently, the anchored peptide is extended by a series of addition cycles (Fig. 18.1. 1).
What is solid-phase synthesis used for?
Solid-phase synthesis is a method used to streamline the synthesis of molecules. It is often used in combinatorial chemistry(a technique used to prepare a large number of molecules in a short period of time), to generate libraries of compounds due to the ease of purification, and overall chemical synthesis.
What are peptides explain Solid Phase Peptide Synthesis?
What are advantages of solid phase peptide synthesis?
General advantages of solid phase synthesis are easy purification, rapid generation of linear peptide intermediates, and precedent in the synthesis of large peptides. Given the ease with which peptide linear precursors are produced on solid phase, we opted to employ this approach in addition to a solution phase method.