What is rat tail collagen?

General description. Rat Tail Collagen Coating Solution (20 ML) enhances cell attachment and proliferation, or promotion of cell-specific morphology and function. It is commonly used for endothelial cells, hepatocytes, muscle cells, and a variety of other cell types.

How do you store collagen coated plates?

Label and date tube, store at 4°C, use within 7 days. Incubate overnight at room temperature to ensure ensure that collagen has adhered to the plastic. If flasks/plates are urgently needed incubate 2-3 hours at 37°C. After the incubation, excess collagen may be taken off and used to coat other surfaces.

How do you dissolve type 1 collagen?

Collagen Type I coating protocol for culture ware Add collagen to acetic acid (refer to Table 1) to obtain 0.1% (w/v) collagen solution. Stir at room temperature for 1-3 hours until dissolved. Collagen solution should be diluted 10-fold to obtain a working concentration of 0.01%.

How do you dilute rat tail collagen?

For your application, the thin coating procedure should be fine at a starting concentration of 5 µg/cm2. You can dilute the collagen in 20 mM acetic acid to the volume needed. Coat the surface, incubate 1 hour at room temp, aspirate the solution, rinse 3 times with PBS, and use or store plates.

What does Type 1 collagen do?

Type I collagen is by far the most abundant protein in all vertebrates. It assembles into fibers that form the structural and mechanical scaffold (matrix) of bone, skin, tendons, cornea, blood vessel walls and other connective tissues.

How do you sterilize collagen?

To achieve long-term durability, the collagen materials are dried and subsequently sterilized. Most common sterilization methods are unsui- table as they cause denaturation. Currently, only ethylenoxide or γ irradiation pre- serves the microscopic and macroscopic collagen structure.

What does collagen dissolve best in?

Collagen generally gets solubilzed in acidic condition. My experiments need collagen to be at neutral pH. But It’s really getting difficult as collagen precipitate when the pH was increased.

What makes collagen dissolve?

Collagen is completely soluble in 0.1 M acetic acid prepared in sterile H2O after steering 1-3 hrs at room temperature. the isoelectric point of collagen is 4.7, and 0.1M acetic acid has the pH=2.88, so collagen in this acid has a positive net charge and is completely soluble.

Which is better type 1 or 2 collagen?

Type 1 collagen is most abundant in Marine Collagen. Type 2 Collagen: Makes up a majority of the protein molecules in your cartilage, the connective tissue that protects your bones at the joints, in your spinal disks, and your eyes, making it a potent way to support joint health.

How much type 1 collagen should I take?

A 2019 review of clinical studies found that taking 2.5–15 grams of hydrolyzed collagen peptides each day may be safe and effective ( 29 ). A smaller daily dose of 2.5 grams could benefit joint pain, skin health, and hydration ( 7 , 30 , 31 ).