What is RPMI 1640 where and for what purpose this chemical is used?
RPMI 1640 has traditionally been used for the serum-free expansion of human lymphoid cells. RPMI 1640 uses a bicarbonate buffering system and differs from most mammalian cell culture media in its typical pH 8 formulation.
Does RPMI 1640 contain HEPES?
RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas….This RPMI 1640 is modified as follows:
| With | Without |
|---|---|
| • Phenol Red | • L-glutamine |
| • HEPES |
Does RPMI 1640 contain glucose?
In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors….This RPMI is modified as follows:
| With | Without |
|---|---|
| • L-glutamine | • Glucose |
| • Phenol Red | • HEPES |
Does RPMI have phenol red?
RPMI 1640 Medium, no phenol red.
What does RPMI-1640 do?
Product Overview. RPMI 1640 Medium was developed for culturing normal human leukocytes. It can be used for suspension and monolayer mammalian cultures. RPMI 1640 Medium will work with different cell lines, it can also be used with primary cells such as blood/hematopoietc cells.
Does RPMI need HEPES?
RPMI is manufactured as enriched medium with or without L-glutamine with a stable L-glutamine substitute, with or without HEPES. RPMI 1640 requires supplementation with serum, typically 10% Fetal Bovine Serum (FBS), since this medium does not contain proteins, lipids, or growth factors.
What is difference between RPMI 1640 and DMEM?
DMEM (low glucose) contains a lower concentration of glucose (1 g/L) than RPMI 1640 (2 g/L). In addition, DMEM has a higher concentration in calcium (1.8 mM) and a lower concentration in phosphate (1 mM) than RPMI 1640 which contains 0.8 mM of calcium and 5 mM of phosphate.